The bachelor thesis deals with the identification and analysis of negative childhood events in the paediatric records of the ELSPAC study participants. The objectives of the thesis include studying the issue of negative events in childhood, identifying neg-ative or traumatic events in the paediatric records of the ELSPAC study, categorizing them according to ACE definitions, and creating descriptive statistics. Finally, to as-sociate the obtained ACEs with substance use in adolescence and to compare with the available literature. In this study, a lower prevalence of ACEs was found compared to other stud-ies. When examining the effect of ACEs on substance use, no association was con-firmed between ACEs and lifetime substance use or between ACEs and past month illicit substance use. The effect of ACE on early onset of cigarettes or alcohol use was statistically significant. This result was consistent with previous studies.