The presented diploma thesis focuses on the sedimentological research of the Lower Miocene sediments of the Czech part of the Vienna Basin. Samples taken from drill cores of MND a.s. were analyzed using lithofacies analysis, X-ray fluorescence spectrometry, grain size analysis, laboratory gamma spectrometry and study of translucent heavy minerals. Sediments of Eggenburgian age most likely represent shallow marine sediments, specifically, it could be the lower part of the Lužice Formation. The studied sediments of the Ottnangian age represent shallow-sea sediments deposited below the rising water level, which are most likely part of the Hodonín sands - the basal clastics of the Ottnangian. The deposition of the studied sediments of Karpatian age most likely took place in marine lagoons. Based on the similarity of the results of analyzes of detrital garnets with the results from the Carpathian Foredeep, Moravicum mica-schist and pararules were considered as the source rocks of the sediments of Karpatian age.