This diploma thesis is dedicated to the national referendum in the Czech Republic. The aim of the thesis is to analyse and evaluate the drafts of acts regulating national referendum. At first I focus on the theoretical questions and define particular terms related to the topic of the thesis. I also mention the national referendum on the accession of the Czech Republic to the European Union. In the most extensive part of the thesis I describe and analyze the drafts of acts regulating national referendum. In the final part I summarize the drafts of acts and I mention how the drafts of acts regulating national referendum should look like.